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  • ICAR and TNAU E-Course Summarized

    Summarized Notes
  • Which of the following serves as primer during reverse transcription of the retroviral genomes?

    Question: Which of the following serves as primer during reverse transcription of the retroviral genomes?

    Options:

    A primer RNA
    A uncharged tRNA
    A nucleotide linked to a protein
    Both (a) and (b)

    ✅Explanation:• The primer for reverse transcription of retroviral genomes is a host tRNA. The tRNA is bound to the viral RNA by the primer binding site (PBS). The reverse transcriptase enzyme then uses the tRNA as a template to synthesize a complementary DNA strand.

    Other options explanations:
    (A) primer RNA
    -A primer RNA is a short piece of RNA that is used to initiate DNA synthesis. However, it is not used by retroviruses.
    (C) A nucleotide linked to a protein
    -A nucleotide linked to a protein is not used as a primer for reverse transcription.

    🔴Related Terminologies::
    -Reverse transcription: The process of synthesizing DNA from an RNA template.
    -tRNA: A type of RNA that is used to transfer amino acids to the ribosome during protein synthesis.
    -Reverse transcriptase: The enzyme that synthesizes DNA from an RNA template.

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