Question: What type of hybridization occurs in a Northern blot experiment using a probe?
Options:
DNA:DNA hybrid
RNA:RNA hybrid
Both a and b
None of these
✅Explanation:
A Northern blot is a molecular biology technique used to detect specific RNA molecules within a sample. It uses a labeled probe, which is a short piece of single-stranded RNA complementary to the target RNA sequence. During the hybridization step, the probe binds to its complementary target RNA molecule in the sample based on Watson-Crick base pairing. This forms a RNA:RNA hybrid between the probe and the target RNA.
A Northern blot is a molecular biology technique used to detect specific RNA molecules within a sample. It uses a labeled probe, which is a short piece of single-stranded RNA complementary to the target RNA sequence. During the hybridization step, the probe binds to its complementary target RNA molecule in the sample based on Watson-Crick base pairing. This forms a RNA:RNA hybrid between the probe and the target RNA.