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  • ICAR and TNAU E-Course Summarized

    Summarized Notes
  • What type of hybridization occurs in a Northern blot experiment using a probe?

    Question: What type of hybridization occurs in a Northern blot experiment using a probe?

    Options:

    DNA:DNA hybrid
    RNA:RNA hybrid
    Both a and b
    None of these

    ✅Explanation:
    A Northern blot is a molecular biology technique used to detect specific RNA molecules within a sample. It uses a labeled probe, which is a short piece of single-stranded RNA complementary to the target RNA sequence. During the hybridization step, the probe binds to its complementary target RNA molecule in the sample based on Watson-Crick base pairing. This forms a RNA:RNA hybrid between the probe and the target RNA.
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