Question: What is the primary product formed at the end of glycolysis?
Options:
Pyruvic acid
Citric acid
Malic acid
Oxalic acid
🛑Aditional Information:
-Glycolysis is a metabolic pathway that converts glucose into pyruvate, releasing energy in the form of two net molecules of adenosine triphosphate (ATP).
-It is an anaerobic process that occurs in the cytoplasm of the cell.
• Step-by-step explanation of the glycolysis process:
Glucose Activation (Preparatory Phase):Â In this phase, glucose is prepared for the subsequent steps, and this phase ends up consuming energy.
-Step 1:Â Phosphorylation of Glucose: Glucose is converted into glucose-6-phosphate by the enzyme hexokinase. This step uses one molecule of ATP, converting it to ADP.
-Step 2:Â Conversion to Fructose-6-phosphate: Glucose-6-phosphate is rearranged into fructose-6-phosphate by the enzyme phosphoglucose isomerase.
-Step 3:Â Phosphorylation of Fructose-6-Phosphate: Fructose-6-phosphate is converted into fructose-1,6-bisphosphate by the enzyme phosphofructokinase. This step uses another molecule of ATP.
-Step 4:Â Cleavage of Fructose-1,6-bisphosphate:Â The enzyme fructose-bisphosphate aldolase splits fructose-1,6-bisphosphate into two three-carbon sugars: glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP).
-Step 5: Conversion of DHAP to G3P: The enzyme triose phosphate isomerase converts dihydroxyacetone phosphate into a second molecule of glyceraldehyde-3-phosphate.
Energy Extraction (Payoff Phase):Â In this phase, the glucose molecule has been split into two 3-carbon compounds, and ATP and NADH are produced when these are further broken down.
-Step 6:Â Oxidation of G3P: Each G3P is converted into 1,3-bisphosphoglycerate (BPG) by the enzyme glyceraldehyde-phosphate dehydrogenase. In this step, one molecule of NAD+Â is reduced to form NADH per G3P.
-Step 7:Â Production of ATP:Â Each BPG molecule is converted into 3-phosphoglycerate (3PG) by the enzyme phosphoglycerate kinase. This step produces one ATP per BPG.
-Step 8: Conversion to 2-phosphoglycerate (2PG): Each 3PG molecule is rearranged into 2PG by the enzyme phosphoglycerate mutase.
-Step 9: Dehydration to form Phosphoenolpyruvate (PEP): Each 2PG molecule is dehydrated to form PEP by the enzyme enolase.
-Step 10:Â Production of Pyruvate and ATP:Â Each PEP is converted into pyruvate by the enzyme pyruvate kinase, producing a second molecule of ATP.