Sign In Question: What does the CT-value (cutoff threshold) in real-time PCR experiments mean?
• Explanation:
-In real-time PCR (qPCR), the CT-value (cycle threshold) is the number of PCR cycles required for the fluorescent signal to cross a defined threshold.
-This threshold indicates the point at which the amplified DNA product is detectable above the background noise.
-A lower CT-value means that fewer cycles were needed to reach the threshold, implying that there was a higher initial amount of target DNA (or cDNA in the case of RT-qPCR) in the sample.
-In other words, a lower CT-value corresponds to a higher abundance of the transcript of interest.
-In real-time PCR (qPCR), the CT-value (cycle threshold) is the number of PCR cycles required for the fluorescent signal to cross a defined threshold.
-This threshold indicates the point at which the amplified DNA product is detectable above the background noise.
-A lower CT-value means that fewer cycles were needed to reach the threshold, implying that there was a higher initial amount of target DNA (or cDNA in the case of RT-qPCR) in the sample.
-In other words, a lower CT-value corresponds to a higher abundance of the transcript of interest.
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