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  • ICAR and TNAU E-Course Summarized

    Summarized Notes
  • The Shine Dalgarno sequence present on mRNA in case of prokaryotes plays an important role in protein synthesis. This sequence binds with _______ during translation initiation

    Question: The Shine Dalgarno sequence present on mRNA in case of prokaryotes plays an important role in protein synthesis. This sequence binds with _______ during translation initiation

    Options:

    16s RNA
    tRNA
    23S RNA
    5s RNA

    • Explanation:
    -The Shine-Dalgarno sequence is a ribosomal binding site in prokaryotic mRNA, generally located around 8 bases upstream of the start codon AUG.  
    -It is complementary to a sequence on the 3' end of the 16S rRNA, which is a component of the small ribosomal subunit (30S).
    -This base pairing between the Shine-Dalgarno sequence and 16S rRNA helps position the ribosome correctly on the mRNA for translation initiation.

    🔑Key Points:
    The TATA box is a DNA sequence commonly found in the core promoter region of genes in eukaryotes and archaea. It serves as a crucial binding site for transcription factors and RNA polymerase II, which are necessary for the initiation of transcription. The consensus sequence for the TATA box is TATAWAW, where "W" represents either adenine (A) or thymine (T).
    -Shine-Dalgarno sequence: Found in prokaryotic mRNA, it is essential for the initiation of translation, not transcription. It helps in the binding of the ribosome to the mRNA.
    -TATA box: As described above, this is the correct option for eukaryotic promoters
    -Pribnow box: This is found in the promoter region of bacterial DNA, analogous to the TATA box in eukaryotes, but with a shorter consensus sequence (TATAAT).
    -3' UTR: This is the 3' untranslated region of mRNA, important for post-transcriptional regulation but not involved in the initiation of transcription

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