Question: The "King of Flowers" is often considered to be: ((MCAER 2014))
Options:
Genetic engineering
DNA sequencing
PCR (Polymerase Chain Reaction)
All of the above
🔑Key Points:
-Restriction enzymes belong to a larger class of enzymes called nucleases.
-Restriction enzymes are used to break DNA molecules.
-Restriction enzymes are of three types-Â Exonuclease, endonuclease, and restriction endonuclease.
-Exonuclease- They cut off nucleotides from 5' or 3' ends of DNA molecule.
-Endonuclease- They break DNA duplex at any point except the end.
-Restriction endonuclease- They cleave DNA duplex at specific points.
-Restriction endonuclease was found by Arber.
-They act as "molecular scissors".
-They recognize the base sequence at palindrome sites in the DNA duplex and cut its strands.
-Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated.
-It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs.
-This specific base sequence is known as the recognition sequence for Hind II.