Select Your Favourite
Category And Start Learning.

  • ICAR and TNAU E-Course Summarized

    Summarized Notes
  • The “King of Flowers” is often considered to be: ((MCAER 2014))

    Question: The "King of Flowers" is often considered to be: ((MCAER 2014))

    Options:

    Genetic engineering
    DNA sequencing
    PCR (Polymerase Chain Reaction)
    All of the above

    ✅Explanation: Restriction endonucleases are enzymes that cut DNA at specific sequences, known as restriction sites. They are essential tools in genetic engineering, DNA sequencing, and PCR.

    🔑Key Points:
    -Restriction enzymes belong to a larger class of enzymes called nucleases.
    -Restriction enzymes are used to break DNA molecules.
    -Restriction enzymes are of three types- Exonuclease, endonuclease, and restriction endonuclease.
    -Exonuclease- They cut off nucleotides from 5' or 3' ends of DNA molecule.
    -Endonuclease- They break DNA duplex at any point except the end.
    -Restriction endonuclease- They cleave DNA duplex at specific points.
    -Restriction endonuclease was found by Arber.
    -They act as "molecular scissors".
    -They recognize the base sequence at palindrome sites in the DNA duplex and cut its strands.
    -Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated.
    -It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs.
    -This specific base sequence is known as the recognition sequence for Hind II.

      0
      Your Cart
      Your cart is emptyReturn to Shop