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  • ICAR and TNAU E-Course Summarized

    Summarized Notes
  • T₂ test colour of living tissues of seed changed to

    Question: T₂ test colour of living tissues of seed changed to

    Options:

    Red
    Blue
    Yellow
    Green

    ✅Explanation: The T₂ test, also known as the tetrazolium test, is a biochemical method used to assess seed viability. In this test, 2,3,5-triphenyl tetrazolium chloride (TTC) is used. Viable seeds with active dehydrogenases (enzymes involved in respiration) reduce the colorless TTC to a red-colored compound called formazan. This color change indicates that the seed is alive and capable of germination.

    🔑Key Points:
    -The tetrazolium chloride test is also knowns as the biochemical test.
    -This test is used for testing the viability of the seed.
    -In this method, seeds are soaked in 0.5 to 2% solution of tetrazolium chloride (2,3, S-triphenyl tetrazolium chloride).
    -The viable or living seed takes bright red coloration which becomes more intense in the embryo while the dead seed remains in its original color.

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