Question: Counter stain used in gram staining –
Options:
Ethyl Alcohol
Iodine solution
Crystal violet
Safranin
Safranin is the counterstain used in Gram staining. After the decolorization step, Gram-negative bacteria lose the initial crystal violet stain. Safranin, a red dye, is then applied to stain these decolorized cells, allowing them to be visualized as pink or red under the microscope.
🔑Key Points:
-Safranin is a natural stain utilized in histology and cytology.
-Safranin is utilized as a counterstain in some staining conventions, shading cell cores red.
-This is the exemplary counterstain in both Gram stains and endospore staining.
-Safranin ordinarily has the synthetic design displayed at the right (at times depicted as dimethyl safranin).
🛑Additional Information
-Red platelets (RBCs), are likewise alluded to as red cells.
-RBCs take up oxygen in the lungs, or in fish the gills, and delivery it into tissues while just barely getting through the body's vessels.
-Cresol red is a triarylmethane color oftentimes utilized for checking the pH in aquaria.
-Phenolphthalein is much of the time utilized as a pointer in corrosive base titrations.
-Methyl orange is a well known pH marker that is utilized in titration.