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  • ICAR and TNAU E-Course Summarized

    Summarized Notes
  • Counter stain used in gram staining –

    Question: Counter stain used in gram staining –

    Options:

    Ethyl Alcohol
    Iodine solution
    Crystal violet
    Safranin

    ✅Explanation:
    Safranin is the counterstain used in Gram staining. After the decolorization step, Gram-negative bacteria lose the initial crystal violet stain. Safranin, a red dye, is then applied to stain these decolorized cells, allowing them to be visualized as pink or red under the microscope.

    🔑Key Points:
    -Safranin is a natural stain utilized in histology and cytology.
    -Safranin is utilized as a counterstain in some staining conventions, shading cell cores red.
    -This is the exemplary counterstain in both Gram stains and endospore staining.
    -Safranin ordinarily has the synthetic design displayed at the right (at times depicted as dimethyl safranin).
    🛑Additional Information
    -Red platelets (RBCs), are likewise alluded to as red cells.
    -RBCs take up oxygen in the lungs, or in fish the gills, and delivery it into tissues while just barely getting through the body's vessels.
    -Cresol red is a triarylmethane color oftentimes utilized for checking the pH in aquaria.
    -Phenolphthalein is much of the time utilized as a pointer in corrosive base titrations.
    -Methyl orange is a well known pH marker that is utilized in titration.

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