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  • ICAR and TNAU E-Course Summarized

    Summarized Notes
  • A CsCl gradient will separate DNA molecules by

    Question: A CsCl gradient will separate DNA molecules by

    Options:

    Absorption
    Resorption
    Density
    Adhesion

    ✅ Explanation:
    -Density gradient centrifugation is a technique where a solution of varying density is created (in this case, using CsCl). When a mixture of molecules is centrifuged in this gradient, each molecule will migrate to a position in the tube where its density matches the density of the surrounding solution. This allows for the separation of molecules based on their density differences.
    -DNA molecules can have slightly different densities based on their base composition (GC content) or the presence of isotopes. CsCl density gradient centrifugation is a powerful technique to separate DNA molecules based on these subtle density differences.
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