The anthrone test, used in carbohydrate analysis, typically results in which color change? (MCAER 2019)

✅Explanation: The anthrone test is a chemical test used to detect the presence of carbohydrates, with a positive result indicated by a color change to blue or blue-green.

🔑Key Points::
-Anthrone Test: A biochemical assay used for the quantitative estimation of carbohydrates.
-Principle: Based on the reaction of carbohydrates with anthrone reagent under acidic conditions, resulting in the formation of a blue-green colored complex.

✏️Steps:
-Hydrolysis of carbohydrates: Polysaccharides or complex sugars are hydrolyzed into simpler sugars.
-Dehydration of monosaccharides: Simple sugars are dehydrated by sulfuric acid to form furfural or its derivatives.
-Condensation with anthrone reagent: Furfural or hydroxymethyl furfural reacts with anthrone reagent, resulting in a blue-green complex.
-Measurement: Absorbance of the resulting solution is measured at 620 nm using a spectrophotometer.